-
-
-
- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
- What You Need to Know about Antibiotic Resistance – Findings, Facts and Good Intentions
- Why Do Old Men Have Big Ears?
- The Condemned Live Longer: A Potential Paradigm Shift in Genetics
- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
- Taskforce Microbots: Targeted Treatment from Inside the Body
- Eyes on Cancer Therapy
-
-
-
-
-
- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
- What You Need to Know about Antibiotic Resistance – Findings, Facts and Good Intentions
- Why Do Old Men Have Big Ears?
- The Condemned Live Longer: A Potential Paradigm Shift in Genetics
- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
- Taskforce Microbots: Targeted Treatment from Inside the Body
- Eyes on Cancer Therapy
-
-
Braking Ramps
Lab Academy
Most of the larger benchtop centrifuges on the market as well as some small benchtop centrifuges offer what are called acceleration and deceleration (braking) ramps. Most small benchtop centrifuges also enable the user to switch between a normal brake and a soft/slow brake.
もっと読む
表示を減らす
To prevent remixing the phases, the protocol requires that acceleration/deceleration be shut off completely so that the centrifuge accelerates slowly and high forces cannot disturb the gradient. During the run, the different blood components can pass through the gradient and accumulate in the appropriate phase according to their density. When the brake is shut off, the centrifuge simply runs down until the rotor stops by itself. This way, the gradient will not be disturbed by sudden braking.
もっと読む
表示を減らす
When pelleting cells, especially yeast or mammalian cells in flat-bottom vessels in a higher volume in a swing-out rotor, the pellet is not completely solid (depending on the application, it may not be possible to spin the cells at a higher speed to get a more compact pellet, because mammalian cells in particular are quite sensitive when it comes to centrifugal forces). The fact that the pellet is not completely solid means that when braking forces are too strong, the pellet could be remixed, leading to sample loss when the supernatant is discarded. Therefore, we recommend lowering the deceleration ramp (usually it is not necessary to shut off the brake completely) to keep the pellet from swirling up. The use of a vessel with a conical bottom shape can also help you get a tighter pellet, even when centrifuging at lower speeds.
Thus centrifuges with different acc/dec ramps offer users the option of adjusting acceleration and deceleration and optimizing their protocols quite easily.